Cells are stimulated mimicking the pathologic stimuli which causes rejection in renal transplant (RT).

The activated cells are put in a 3D culture (hydrogel) into channeled wells and then exposed to a panel of immunosuppressants.

A drug concentration gradient is created in the channels and over the cells. Drugs are expected to inhibit activation and proliferation of cells. The more drug concentration, the more effect over the cells is expected.

Cell activation/proliferation after drug exposure is captured by a vital probe and read by a fluorimeter, which raw data is uploaded to a portal where our cloud-based software captures the dose/response effect, by calculating and analyzing typical pharmacological dose/response curves.

The bioassay offers precise and reliable dose-response curves.

The software includes algorithms to study the dose-response curves and to position the patient in the reference population (Biohope’s database)